Purpose

Chemo- proteomics has recently become a great Mass Spectrometry based tool for protein drug targets and off-targets identification and to date there are no easy-to-use data analysis pipelines.

Results

We have created a Shiny-based Web application named DOSCHEDA (Down Stream Chemo-Proteomics Data Analysis), to rapidly assess the quality of the chemo-proteomics experiments and perform statistical analysis for extraction of the most competed proteins.DOSCHEDA is designed to be used with minimal user input and requires no programming, increasing its accessibility; it is MS-software independent that is Proteome Discover or MaxQuant outputs are, but not restricted to, accepted input types. DOSCHEDA aggregates results from bioinformatics analyses of dose-response chemo-proteomics experiments into a dynamic interface which includes interactive graphics and enables customized output reports.

Instructions

For detailed instructions on how to use DOSCHEDA please refer to the manual and walkthroughs available at the GitHub https://github.com/brunocontrino/DOSCHEDA

Availability

DOSCHEDA is implemented entirely in the R language. It can be hosted/launched by any system with R installed, including Windows, Mac OS and most Linux distributions. Information technology administrators can also host DOSCHEDA from a remote server, in which case users need only have a Web browser installed. DOSCHEDA is provided free of charge under a GPL-3 open-source license through GitHub