Author: ampliCan

# Report breakdown by barcode

# Description

Read distribution plot - plot shows number of reads assigned during read grouping
Filtered Reads - plot shows percentage of assigned reads that have been recognized as PRIMER DIMERS or filtered based on low alignment score
Edit rates - plot gives overview of percentage of reads (not filtered as PRIMER DIMER) that have edits
Frameshift - plot shows what percentage of reads that have frameshift
Top unassigned reads - take a look at the alignment of most abundant forward and reverse complemented reverse reads for each barcode, if you find that there is many unassigned reads you can ivestigate here.

# Barcode Summary

## Groups IDs

group | IDs
--- | ---
barcode_1 | ID_1, ID_2
barcode_2 | ID_3, ID_4, ID_5

## Read distribution

## Filtered Reads

## Edit rates

## Frameshift

# Top unassigned reads

## barcode_1

Forward | Reverse | Counts | Frequency
--- | --- | --- | ---
P1 | S1 | 1 | 0.0588235
P2 | S2 | 1 | 0.0588235
P3 | S3 | 1 | 0.0588235
P4 | S4 | 1 | 0.0588235

    P1                 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTGTTTTGATGATAAA     50
                         ||   ||| |    | | |||   |   ||    |||          |||
    S1                 1 AAG--CTGAC----GGCTAAA---TGAAAAATATCTG----------AAA     31

    P1                51 CTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTT--GGCAGAG-----G     93
                         | |       || ||  ||  ||||  | | ||    |||||||     |
    S1                32 CAT-------CTGTTCCAG--GTGCTGCGTATGCCAGGGCAGAGAAGAAG     72

    P1                94 GTCAGC--AGACCAGT--AAGTCTTCTCAATTTCTTTTATTT----ATGT    135
                         |||||   ||  || |  | |||      ||  | ||| ||     |
    S1                73 GTCAGGGAAGGTCACTGGAGGTCACTGGGATACCCTTTCTTCCCACACCA    122

    P1               136 AT-----ATGTAGT-------GATAAA-A    151
                         ||     | | |||       ||| |  |
    S1               123 ATGGGGAAAGGAGTCCTGCCAGATGACCA    151

    P2                 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTG---TTTTGATGAT     47
                                 | || | || | |  | | |||      |   ||  || ||
    S2                 1 --------TTTTATCACTACATATACATAAATAAAAGAAATTGAGAAGAC     42

    P2                48 NNNCTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTTGGCAGAGGGTCA     97
                              ||| ||  ||  ||    | ||||         |||| |    ||
    S2                43 -----TTACTGGTCTGCTGACCCTCTGCCAACACAAGGGGCACAACTACA     87

    P2                98 GCAGACCAGTAAG-----TCTTCTCAATTTCTTTTATTTATGTATATGTA    142
                           ||  || |||      || ||  ||   |   |   || | |  | |
    S2                88 AAAGCACAATAAAGNNNATCATCAAAACAGCACCT---TAAGAAGGTTTG    134

    P2               143 GTGATAAAA--------    151
                         || | || |
    S2               135 GTCACAAGACAGTATTT    151

    P3                 1 AAGCTGACGGCTAAATNNNAAATATCTGAAACATCTGTTCCAGGTGCTGC     50
                         ||||||||||||||||   |||||||||||||||||||||||||||||||
    S3                 1 AAGCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50

    P3                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S3                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100

    P3               101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC    150
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S3               101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC    150

    P3               151 A    151
                         |
    S3               151 A    151

    P4                 1 AAGCTNGCGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50
                            ||  |||||||||||||||||||||||||||||||||||||||||||
    S4                 1 NNNCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50

    P4                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S4                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100

    P4               101 GATACCCTTTCTTCCCACACCANNNGGGAAAGGAGTCCTGCCAGATGACC    150
                         |||||||   ||||||||||||   ||||||||||||||    |||||||
    S4               101 GATACCCNNNCTTCCCACACCAATGGGGAAAGGAGTCCTNNNNGATGACC    150

    P4               151 A    151
                         |
    S4               151 A    151