Author: Daniele Ramazzotti
Date: 2026-04-28

# Introduction

Daniele Ramazzotti, Fabrizio Angaroni, Davide Maspero, Alex Graudenxi, Luca De Sano and Gianluca Ascolani

#### April 28, 2026

#### Package

LACE 2.16.0

## 1 Overview

LACE is an algorithmic framework that processes single-cell somatic mutation profiles from cancer samples collected at different time points and in distinct experimental settings, to produce longitudinal models of cancer evolution. The approach solves a Boolean Matrix Factorization problem with phylogenetic constraints, by maximizing a weighted likelihood function computed on multiple time points.

## 2 Installing LACE

The R version of LACE can be installed from BioConductor. To do so, we need to install the R packages LACE depends on and the devtools package.

    if (!require("BiocManager", quietly = TRUE))
        install.packages("BiocManager")

    BiocManager::install("LACE")

## 3 Debug

Please feel free to contact us if you have problems running our tool at daniele.ramazzotti1@gmail.com or d.maspero@campus.unimib.it.