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scOverlay

This is the development version of scOverlay; to use it, please install the devel version of Bioconductor.

Multilayer Plots for Single-Cell Data


Bioconductor version: Development (3.24)

scOverlay provides publication-grade plotting helpers to display a foreground feature (gene expression or metadata) on top of a contextual background (clusters, cell types, samples, or a solid colour) in the same reduced dimension panel, each with independent colour scales (via ggnewscale). It integrates with SingleCellExperiment / SummarizedExperiment, supports flexible subsetting of foreground cells, multiple assays (e.g. counts, logcounts), and grid arrangements by user-defined grouping columns.

Author: Bernat Gel [aut, cre] ORCID iD ORCID: 0000-0001-8878-349X

Maintainer: Bernat Gel <bgel at igtp.cat>

Citation (from within R, enter citation("scOverlay")):
Seminal Bioconductor project articles:

Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M (2015). "Orchestrating high-throughput genomic analysis with Bioconductor." Nature Methods, 12(2), 115–121. doi:10.1038/nmeth.3252.

Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J (2004). "Bioconductor: open software development for computational biology and bioinformatics." Genome Biology, 5(10), R80. doi:10.1186/gb-2004-5-10-r80.

Installation

To install this package, start R (version "4.6") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")

BiocManager::install("scOverlay")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("scOverlay")
scOverlay: layered visualization of single-cell embeddings HTML R Script
Reference Manual PDF

Details

biocViews DataRepresentation, GeneExpression, SingleCell, Software, Transcriptomics, Visualization
Version 0.99.5
In Bioconductor since BioC 3.24 (R-4.6)
License Artistic-2.0
Depends R (>= 4.6.0)
Imports ggplot2, ggnewscale, SummarizedExperiment, SingleCellExperiment, viridisLite, scales, RColorBrewer, patchwork, ggrastr, rlang
System Requirements
URL https://github.com/bernatgel/scOverlay
Bug Reports https://github.com/bernatgel/scOverlay/issues
See More
Suggests knitr, rmarkdown, testthat (>= 3.0.0), BiocStyle
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package scOverlay_0.99.5.tar.gz
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64) scOverlay_0.99.5.tgz
macOS Binary (sonoma-arm64)
Source Repository git clone https://git.bioconductor.org/packages/scOverlay
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/scOverlay
Bioc Package Browser https://code.bioconductor.org/browse/scOverlay/
Package Short Url https://bioconductor.org/packages/scOverlay/
Package Downloads Report Download Stats